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IL-12 Protein, Mouse, Recombinant (His & hFc)

Catalog No. TMPY-02351
Synonyms: Il-12b, Il12p40, interleukin 12A, Ll12a, Il-12p40, p35, p40, IL-12p35, Il-12a

IL-12 Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 85.9 kDa and the accession number is P43431&P43432.

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IL-12 Protein, Mouse, Recombinant (His & hFc)
Pack Size Availability Price/USD Quantity
50 μg 5 days $ 618.00
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Biological Description
Technical Params
Product Properties
Description IL-12 Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 85.9 kDa and the accession number is P43431&P43432.
Species Mouse
Expression System HEK293 Cells
Tag C-His, C-hFc
Accession Number P43431&P43432
Synonyms Il-12b, Il12p40, interleukin 12A, Ll12a, Il-12p40, p35, p40, IL-12p35, Il-12a
Construction The p35 subunit of Mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The p40 subunit of Mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of Human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Protein Purity > 85 % as determined by SDS-PAGE
Molecular Weight 85.9 kDa (predicted)
Endotoxin < 1.0 EU/μg of the protein as determined by the LAL method.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice.

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Please read the User Guide of Recombinant Proteins for more specific information.

Keywords

IL-12 Protein, Mouse, Recombinant (His & hFc) Il-12b Il12p40 interleukin 12A Ll12a Il-12p40 p35 p40 IL-12p35 Il-12a recombinant recombinant-proteins proteins protein

 

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