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Anti-VEGF165 Polyclonal Antibody
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Catalog No. TMAB-01947
Alias VPF, VEGFA, VEGF, Vascular permeability factor, L-VEGF
Anti-VEGF165 Polyclonal Antibody is a Rabbit antibody targeting VEGF165. Anti-VEGF165 Polyclonal Antibody can be used in ELISA,WB.
Anti-VEGF165 Polyclonal Antibody
Anti-VEGF165 Polyclonal Antibody
😃Good
Catalog No. TMAB-01947Alias VPF, VEGFA, VEGF, Vascular permeability factor, L-VEGF
Anti-VEGF165 Polyclonal Antibody is a Rabbit antibody targeting VEGF165. Anti-VEGF165 Polyclonal Antibody can be used in ELISA,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $373 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-VEGF165 Polyclonal Antibody is a Rabbit antibody targeting VEGF165. Anti-VEGF165 Polyclonal Antibody can be used in ELISA,WB. |
| Synonyms | VPF, VEGFA, VEGF, Vascular permeability factor, L-VEGF |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 25 μg total protein per Lane of various lysates probed with VEGF165 polyclonal antibody, unconjugated (TMAB-01947) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.  |
| Application | |
| Recommended Dose | WB: 1:500-2000; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. |
| Tissue Specificity | soform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature). |
| Immunogen | Recombinant Protein: human VEGF165 Protein |
| Antigen Species | Human |
| Gene Name | VEGFA |
| Gene ID | |
| Protein Name | Vascular endothelial growth factor A |
| Uniprot ID | |
| Biology Area | VEGF,SARS Coronavirus,Cancer,Hypoxia,Co-factors,Angiogenesis and vasculogenesis,VEGF,Angiogenic growth factors,VEGF,Response to hypoxia |
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| Molecular Weight | Theoretical: 22 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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