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Anti-IL-2 R gamma/CD132 Antibody (3J53)
🥰Excellent
Catalog No. TMAY-03091
Anti-IL-2 R gamma/CD132 Antibody (3J53) is a Rabbit antibody targeting IL-2 R gamma/CD132. Anti-IL-2 R gamma/CD132 Antibody (3J53) can be used in ELISA(Cap),FCM,ICC/IF.
Anti-IL-2 R gamma/CD132 Antibody (3J53)
Anti-IL-2 R gamma/CD132 Antibody (3J53)
🥰Excellent
Catalog No. TMAY-03091
Anti-IL-2 R gamma/CD132 Antibody (3J53) is a Rabbit antibody targeting IL-2 R gamma/CD132. Anti-IL-2 R gamma/CD132 Antibody (3J53) can be used in ELISA(Cap),FCM,ICC/IF.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-IL-2 R gamma/CD132 Antibody (3J53) is a Rabbit antibody targeting IL-2 R gamma/CD132. Anti-IL-2 R gamma/CD132 Antibody (3J53) can be used in ELISA(Cap),FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3J53 |
| Reactivity | Mouse |
| Verified Activity | 1. Immunofluorescence staining of mouse IL2RG in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse IL2RG monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). 2. Flow cytometric analysis of Mouse IL2RG(CD132) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse IL2RG(CD132), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA(Cap),FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD132 / IL2RG (rM CD132 / IL2RG; TMPY-01837; NP_038591.1; Met1-Ala263). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (γc) (or IL2RG), is a cytokine receptor subunit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15, and the interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (X-SCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor based on chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why a deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency. |
| Conjucates | Unconjugated |
| Others Formats | PE |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Mouse CD132 / IL2RG Protein (TMPY-01837) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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