Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, FCM

Reactivity: Human

Rabbit IgG

9B532

Human

1. Western Blot
-Positive WB detected in: HEK293 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, A549 whole cell lysate
-All lanes: RPS19 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 17 kDa
-Observed band size: 15-20 kDa
2. Overlay Peak curve showing HepG2 cells stained with TMAH-01052 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FCM

WB:1:500-1:2000; FCM:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human RPS19

Human

6223

Epigenetics and Nuclear Signaling