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Anti-BRAF Antibody (7G224)

Catalog No. TMAB-03199 Copy Product Info
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Anti-BRAF Antibody (7G224) is a Mouse antibody targeting BRAF. Anti-BRAF Antibody (7G224) can be used in WB,IHC-P,IHC-Fr,IF,ELISA,IP.

Anti-BRAF Antibody (7G224)

Copy Product Info
🥰Excellent
Catalog No. TMAB-03199

Anti-BRAF Antibody (7G224) is a Mouse antibody targeting BRAF. Anti-BRAF Antibody (7G224) can be used in WB,IHC-P,IHC-Fr,IF,ELISA,IP.

Anti-BRAF Antibody
(7G224)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2237-10 days7-10 days
100 μL$3717-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-BRAF Antibody (7G224) is a Mouse antibody targeting BRAF. Anti-BRAF Antibody (7G224) can be used in WB,IHC-P,IHC-Fr,IF,ELISA,IP.
Ig Type
IgG2a, κ
Clone
7G224
Reactivity
Human,Mouse,Rat
Verified Activity
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Human Raji cell lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human MOLT4 cell lysates
Lane 7: Human HL-60 cell lysates
Primary: Anti-B-Raf (TMAB-03199) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 84 (hu)/89 (mo, rat) kDa
Observed band size: 95 (hu)/100 (mo, rat) kDa
Application
WB
IHC-P
IHC-Fr
IF
ELISA
IP
Recommended Dose
WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200; ELISA: 1:30-3000; IP: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationNucleus. Cytoplasm. Cell membrane.
Tissue SpecificityBrain and testis.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]
Antigen Details
Immunogen
Recombinant Protein: human B-Raf protein
Antigen Species
Human
Gene Name
BRAF
Gene ID
673
Protein Name
Serine/threonine-protein kinase B-raf
Uniprot ID
P15056
Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
Chemical Properties
Molecular WeightTheoretical: 84 (human)/89 (mouse, rat) kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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