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Anti-TACC3 Antibody (5C664) is an antibody targeting TACC3. Anti-TACC3 Antibody (5C664) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Anti-TACC3 Antibody (5C664) is an antibody targeting TACC3. Anti-TACC3 Antibody (5C664) can be used in ELISA, WB, IHC. |
Alias | Transforming acidic coiled-coil-containing protein 3, TACC 3, ERIC-1, ERIC1 |
Ig Type | Rabbit IgG |
Clone | 5C664 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, THP-1 whole cell lysate -All lanes: TACC3 Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 91 kDa -Observed band size: 140 kDa 2. IHC image of TMAH-01154 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human TACC3 |
Antigen Species | Human |
Gene ID | 10460 |
Uniprot ID | |
Biology Area | Cell biology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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