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Anti-TACC3 Antibody (5C664)

😃Good
Catalog No. TMAH-01154
Alias Transforming acidic coiled-coil-containing protein 3, TACC 3, ERIC-1, ERIC1

Anti-TACC3 Antibody (5C664) is an antibody targeting TACC3. Anti-TACC3 Antibody (5C664) can be used in ELISA, WB, IHC.

Anti-TACC3 Antibody (5C664)

Anti-TACC3 Antibody (5C664)

😃Good
Catalog No. TMAH-01154Alias Transforming acidic coiled-coil-containing protein 3, TACC 3, ERIC-1, ERIC1
Anti-TACC3 Antibody (5C664) is an antibody targeting TACC3. Anti-TACC3 Antibody (5C664) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Anti-TACC3 Antibody (5C664) is an antibody targeting TACC3. Anti-TACC3 Antibody (5C664) can be used in ELISA, WB, IHC.
AliasTransforming acidic coiled-coil-containing protein 3, TACC 3, ERIC-1, ERIC1
Ig Type
Rabbit IgG
Clone
5C664
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: K562 whole cell lysate, THP-1 whole cell lysate
-All lanes: TACC3 Antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 91 kDa
-Observed band size: 140 kDa
2. IHC image of TMAH-01154 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundPlays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human TACC3
Antigen Species
Human
Gene ID
10460
Uniprot ID
Biology Area
Cell biology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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