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Anti-NPR2 Antibody (6D108)

😃Good
Catalog No. TMAH-00824

Anti-NPR2 Antibody (6D108) is an antibody targeting NPR2. Anti-NPR2 Antibody (6D108) can be used in ELISA, WB, IF, FCM.

Anti-NPR2 Antibody (6D108)

Anti-NPR2 Antibody (6D108)

😃Good
Catalog No. TMAH-00824
Anti-NPR2 Antibody (6D108) is an antibody targeting NPR2. Anti-NPR2 Antibody (6D108) can be used in ELISA, WB, IF, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IF, FCM

Reactivity: Human
Ig Type
Rabbit IgG
Clone
6D108
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: HEPG2 whole cell lysate, MCF7 whole cell lysate
-All lanes: ANPRB antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 117 kDa
-Observed band size: 117 kDa
2. Immunofluorescence staining of Hela with TMAH-00824 at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 501-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Overlay Peak curve showing Hela cells surface stained with TMAH-00824 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, WB, IF, FCM
Recommended Dose
WB:1:500-1:2000; IF:1:50-1:200; FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundReceptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human NPR2
Antigen Species
Human
Gene ID
4882
Uniprot ID
Biology Area
Neuroscience, Cardiovascular, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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