PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

1. Loaded Mouse PD-1-His on HIS1K Biosensor, can bind Biotinylated Human PD-L1-Fc-Avi with an affinity constant of <10^-3 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Recombinant Human PD-1-His at 5μg/ml (100 μl/well)can bind Human PD-L1-Fc-Avi. The ED50 of Human PD-L1-Fc-Avi is 0.185 ug/ml. (Regularly tested)

PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 70-95 KDa and the accession number is Q9NZQ7.

Human

HEK293 Cells

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.