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PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated

(Synonyms: Programmed death ligand 1, Programmed Cell Death 1 Ligand 1, PD-L1, PDL1, PDCD1LG1, PDCD1L1, PDCD1 ligand 1, CD274, B7-H1, B7H1, B7 homolog 1) Copy Product Info

Synonyms: Programmed death ligand 1, Programmed Cell Death 1 Ligand 1, PD-L1, PDL1, PDCD1LG1, PDCD1L1, PDCD1 ligand 1, CD274, B7-H1, B7H1, B7 homolog 1

Catalog No. TMPJ-00182 Copy Product Info
PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 70-95 KDa and the accession number is Q9NZQ7.

Batch Information

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Purity:Greater than 95% as determined by reducing SDS-PAGE.
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
1. Loaded Mouse PD-1-His on HIS1K Biosensor, can bind Biotinylated Human PD-L1-Fc-Avi with an affinity constant of <10-3 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Recombinant Human PD-1-His at 5μg/ml (100 μl/well)can bind Human PD-L1-Fc-Avi. The ED50 of Human PD-L1-Fc-Avi is 0.185 ug/ml. (Regularly tested)
Description
PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Fc-Avi tag. The predicted molecular weight is 70-95 KDa and the accession number is Q9NZQ7.
Species
Human
Expression System
HEK293 Cells
TagC-hFc-Avi
Accession NumberQ9NZQ7
Amino AcidPhe19-Thr239
ConstructionPhe19-Thr239
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 150 mM NaCl, pH 7.4.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsProgrammed death ligand 1, Programmed Cell Death 1 Ligand 1, PD-L1, PDL1, PDCD1LG1, PDCD1L1, PDCD1 ligand 1, CD274, B7-H1, B7H1, B7 homolog 1
Research Background
Chemical Properties
Molecular Weight70-95 KDa (reducing condition)
Storage & Solubility Information
StorageStore at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated chemical structure | PD-L1 Protein, Human, Recombinant (hFc & Avi), Biotinylated molecular weight