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Anti-V5 tag Antibody (9T763) (Synonyms: V5tag)

Catalog No. TMAS-00145 Copy Product Info
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Anti-V5 tag Antibody (9T763) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (9T763) can be used in ELISA, FCM, ICC/IF, IP, WB.

Anti-V5 tag Antibody (9T763)

Copy Product Info
🥰Excellent
Catalog No. TMAS-00145
Synonyms V5tag

Anti-V5 tag Antibody (9T763) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (9T763) can be used in ELISA, FCM, ICC/IF, IP, WB.

Anti-V5 tag Antibody
(9T763)
Pack SizePriceUSA StockGlobal StockQuantity
100 μg$5547-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-V5 tag Antibody (9T763) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (9T763) can be used in ELISA, FCM, ICC/IF, IP, WB.
SynonymsV5tag
Ig Type
IgG2a,κ
Clone
9T763
Specificity
C-terminal, N-terminal, and internal V5 tagged fusion proteins
Verified Activity
1. Sensitivity comparison of purified internal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG
2. Immunocytochemistry/Immunofluenrescence analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
3. Comparison sensitivity of purified N-terminal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG
4. Flow cytometric analysis of non-transfected or V5 fusion gene transfected CHO cells using Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
5. Flow cytometric analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with FITC conjugated Goat Anti-Mouse IgG.
6. Western blot analysis of immunoprecipitates from V5-tagged protein transfected HEK293 cell lysates using Anti-V5 tag Antibody (9T763) (TMAS-00145).
Lane 1: V5-tagged protein transfected HEK293 cell lysate as control.
Lane 2-3: Immunoprecipitates of V5-tagged protein transfected and non-transfected HEK293 cell lysate with Anti-V5 tag Antibody (9T763) (TMAS-00145).
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.
7. Sensitivity comparison of purified internal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.
8. Superior specificity assay of transfected cell lysates using Western blot with Anti-V5 tag Antibody (9T763) (TMAS-00145).
Lane 1: V5-tagged protein transfected CHO cell lysate.
Lane 2: Non-transfected CHO cell lysate.
The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG.
Application
Recommended Dose
ELISA: 0.05-0.1 µg/ml; FCM: 1-3 µg for 1 x 10 6 cells; ICC/IF: 1-3 µg/ml; IP: 1-5 µg/ml; WB: 0.2-1 µg/ml
Antibody Type
Monoclonal
Host SpeciesMouse
PurificationProtein A affinity column
AppearanceLyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
FormulationLyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Research BackgroundThe V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
HRP/Biotin/iFluor 488/iFluor 555/iFluor 647
Antibody Types Available
6
Antigen Details
Immunogen
KLH conjugated synthetic peptide: GKPIPNPLLGLDST
Chemical Properties
Stability & Storage
Stability & StorageThe lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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