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Anti-V5 tag Antibody (9T763)
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Catalog No. TMAS-00145
Alias V5tag
Anti-V5 tag Antibody (9T763) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (9T763) can be used in ELISA, FCM, ICC/IF, IP, WB.
Anti-V5 tag Antibody (9T763)
Anti-V5 tag Antibody (9T763)
😃Good
Catalog No. TMAS-00145Alias V5tag
Anti-V5 tag Antibody (9T763) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (9T763) can be used in ELISA, FCM, ICC/IF, IP, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μg | $554 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-V5 tag Antibody (9T763) is a Mouse antibody targeting V5 tag. Anti-V5 tag Antibody (9T763) can be used in ELISA, FCM, ICC/IF, IP, WB. |
| Synonyms | V5tag |
| Ig Type | IgG2a,κ |
| Clone | 9T763 |
| Specificity | C-terminal, N-terminal, and internal V5 tagged fusion proteins |
| Verified Activity | 1. Sensitivity comparison of purified internal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG 2. Immunocytochemistry/Immunofluenrescence analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using Anti-V5 tag Antibody (9T763) (TMAS-00145). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. 3. Comparison sensitivity of purified N-terminal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG 4. Flow cytometric analysis of non-transfected or V5 fusion gene transfected CHO cells using Anti-V5 tag Antibody (9T763) (TMAS-00145). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. 5. Flow cytometric analysis of non-transfected CHO cells (a) and V5 fusion gene transfeced CHO cells (b) using Anti-V5 tag Antibody (9T763) (TMAS-00145). The signal was developed with FITC conjugated Goat Anti-Mouse IgG. 6. Western blot analysis of immunoprecipitates from V5-tagged protein transfected HEK293 cell lysates using Anti-V5 tag Antibody (9T763) (TMAS-00145). Lane 1: V5-tagged protein transfected HEK293 cell lysate as control. Lane 2-3: Immunoprecipitates of V5-tagged protein transfected and non-transfected HEK293 cell lysate with Anti-V5 tag Antibody (9T763) (TMAS-00145). The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG. 7. Sensitivity comparison of purified internal V5 tagged fusion proteins with Anti-V5 tag Antibody (9T763) (TMAS-00145) and Invitrogen (R960-25) by Western blot. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG. 8. Superior specificity assay of transfected cell lysates using Western blot with Anti-V5 tag Antibody (9T763) (TMAS-00145). Lane 1: V5-tagged protein transfected CHO cell lysate. Lane 2: Non-transfected CHO cell lysate. The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG. |
| Application | |
| Recommended Dose | ELISA: 0.05-0.1 µg/ml; FCM: 1-3 µg for 1 x 10 6 cells; ICC/IF: 1-3 µg/ml; IP: 1-5 µg/ml; WB: 0.2-1 µg/ml |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Purification | Protein A affinity column |
| Appearance | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| Formulation | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Research Background | The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). |
| Conjucates | Unconjugated |
| Others Formats | HRP/Biotin/iFluor 488/iFluor 555/iFluor 647 |
| Antibody Types Available | 6 |
| Immunogen | KLH conjugated synthetic peptide: GKPIPNPLLGLDST |
Stability & Storage
| Stability & Storage | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks or at -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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