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Anti-S100A8 Antibody (6E414)
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Catalog No. TMAH-01062
Alias S100 calcium binding protein A8, P8, NIF, MRP-8, MRP8, MIF, MA387, L1Ag, CP-10, CGLA, CFAG, CAGA, 60B8AG
Anti-S100A8 Antibody (6E414) is a Mouse antibody targeting S100A8. Anti-S100A8 Antibody (6E414) can be used in ELISA, IHC.
Anti-S100A8 Antibody (6E414)
Anti-S100A8 Antibody (6E414)
😃Good
Catalog No. TMAH-01062Alias S100 calcium binding protein A8, P8, NIF, MRP-8, MRP8, MIF, MA387, L1Ag, CP-10, CGLA, CFAG, CAGA, 60B8AG
Anti-S100A8 Antibody (6E414) is a Mouse antibody targeting S100A8. Anti-S100A8 Antibody (6E414) can be used in ELISA, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μg | $208 | 7-10 days | |
| 100 μg | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, IHC Reactivity: Human |
| Alias | S100 calcium binding protein A8, P8, NIF, MRP-8, MRP8, MIF, MA387, L1Ag, CP-10, CGLA, CFAG, CAGA, 60B8AG |
| Ig Type | IgG1 |
| Clone | 6E414 |
| Reactivity | Human |
| Verified Activity | 1. Immunohistochemical of paraffin-embedded human cervical cancer using TMAH-01062 at dilution of 1:200 2. Immunohistochemical of paraffin-embedded human spleen using TMAH-01062 at dilution of 1:200 |
| Application | ELISA, IHC |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Secreted. Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation or endothelial adhesion of monocytes, is secreted via a microtubule-mediated, alternative pathway. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Research Background | S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity. (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human S100A8 |
| Antigen Species | Human |
| Gene ID | 6279 |
| Uniprot ID | |
| Biology Area | Neuroscience |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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