NGAL/Lipocalin-2 Protein, Human, Recombinant (hFc)

Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.

Pack Size	Availability	Price/USD
100 μg	5 days	$ 302.00
500 μg	5 days	$ 1,210.00
1 mg	5 days	$ 2,010.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 302.00

500 μg

5 days

$ 1,210.00

1 mg

5 days

$ 2,010.00

Description

Species	Human
Expression System	HEK293
Tag	C-hFc
Accession Number	P80188-1
Synonyms	NGAL, Lipocalin-2, Oncogene 24p3, MSFI, Siderocalin LCN2, p25
Construction	Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln21-Gly198.[Accession \|P80188-1]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in 50mM MES,150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES,150mM NaCl (pH 6.5).
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.