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CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc)
(Synonyms: TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154) Copy Product InfoSynonyms: TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154
Catalog No. TMPK-00860 Copy Product Info
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $48 | - | In Stock | |
| 10 μg | $76 | 7-10 days | 7-10 days | |
| 20 μg | $122 | 7-10 days | 7-10 days | |
| 50 μg | $236 | 7-10 days | 7-10 days | |
| 100 μg | $393 | - | In Stock | |
| 200 μg | $712 | 7-10 days | 7-10 days | |
| 500 μg | $1,570 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA. |
| Description | CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) is expressed in HEK293 mammalian cells with N-monomeric hFc tag. The predicted molecular weight is 75.6 kDa and the accession number is P27548. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | N-monomeric hFc |
| Accession Number | P27548 |
| Construction | Met112-Leu260 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 200 mM L-arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | TRAP, TNFSF5IMD3, TNFSF5, T-BAM, IMD3, IGM, HIGM1, gp39, CD40LIGM, CD40LG, CD40L, CD40 Ligand, CD154 |
| Research Background |
| Molecular Weight | 75.6 kDa (predicted). Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result. |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) chemical structure | CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) molecular weight
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