CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 394.00 | |
500 μg | 5 days | $ 1,570.00 | |
1 mg | 5 days | $ 2,640.00 |
Description | CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
Species | Mouse |
Expression System | HEK293 |
Tag | N-monomeric hFc |
Accession Number | P27548 |
Synonyms | IMD3, CD40LG, CD40L, IGM, TRAP, T-BAM, gp39, HIGM1, CD154, CD40LIGM, CD40 Ligand, TNFSF5, TNFSF5IMD3 |
Construction | Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus. It contains Met112-Leu260.[Accession |P27548] |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
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Please read the User Guide of Recombinant Proteins for more specific information.
CD40 Ligand Protein, Mouse, Recombinant (monomeric hFc) HIGM-1 IMD 3 HIGM 1 CD 154 TNFSF 5 IMD3 CD40LG CD40L IGM TRAP T-BAM IMD-3 gp39 HIGM1 CD154 TNFSF-5 CD40LIGM CD-154 CD40 Ligand TNFSF5 TNFSF5IMD3 recombinant recombinant-proteins proteins protein