Immobilized Human FLT3 Ligand, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 46.0 ng/ml determined by ELISA.
The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is < 1.0 ng/ml. (QC Test)

FLT3 Ligand Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P49771-1.

Human

E. coli

fms related tyrosine kinase 3 ligand,FLT3LG,FLT3L,FL

Thr27-Ala181

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.