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Anti-MMP-9 Polyclonal Antibody 3 is a Rabbit antibody targeting MMP-9. Anti-MMP-9 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,ELISA Reactivity: Human |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. 25 μg total protein per Lane of various lysates probed with MMP9 polyclonal antibody, unconjugated (TMAB-01159) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Polyclonal Antibody, Unconjugated (TMAB-01159) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Polyclonal Antibody, Unconjugated (TMAB-01159) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Polyclonal Antibody, Unconjugated (TMAB-01159) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Polyclonal Antibody, Unconjugated (TMAB-01159) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MMP9 Polyclonal Antibody, Unconjugated (TMAB-01159) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF,ELISA |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted; extracellular space; extracellular matrix. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]. |
Immunogen | KLH conjugated synthetic peptide: human MMP9 |
Antigen Species | Human |
Gene ID | 4318 |
Uniprot ID | |
Biology Area | MMP,Protease inhibitors ELISA kits,Cancer proteins ELISA kits,Atherosclerotic proteins ELISA kits,MMPs,Thrombosis,MMP,MMPs,MMPs,MMPs |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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