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CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated
(Synonyms: T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)) Copy Product InfoSynonyms: T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
Catalog No. TMPY-05374 Copy Product Info
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $91 | 7-10 days | 7-10 days | |
| 10 μg | $148 | 7-10 days | 7-10 days | |
| 20 μg | $245 | - | In Stock | |
| 50 μg | $393 | 7-10 days | 7-10 days | |
| 100 μg | $589 | 7-10 days | 7-10 days | |
| 200 μg | $1,000 | 7-10 days | 7-10 days | |
| 500 μg | $2,030 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 98.4%; SEC-HPLC: 98.4%
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein, Biotinylated (Cat#TMPY-05374) at 2 μg/mL (100 μl/well) on Streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD3 antibody, Mouse IgG2a (Muromonab), the EC50 is 1-3 ng/mL. 2.Amine-Labeled Biotinylated Recombinant Human CD3D & CD3E Heterodimer Protein, Flag & His Tag (Cat#TMPY-05374) captured on CM5 chip via Anti-His antibody, can bind OKT3 derived Anti-CD3 antibody, Mouse IgG2a with an affinity constant of 0.241 μM as determined in an SPR assay (Biacore T200) (Routinely tested). |
| Description | CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-Flag, C-His |
| Accession Number | P04234&P07766 |
| Construction | A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. The purified protein was biotinylated in vitro. Predicted N terminal: Phe 22 & Asp 23 |
| Protein Purity | > 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.   |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex) |
| Molecular Weight | 31.05 kDa (predicted) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated chemical structure | CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated molecular weight
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