TROP-2 Protein, Human, Recombinant (His)

Name: TROP-2 Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01262
Price: 172 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: tumor-associated calcium signal transducer 2, TROP-2, TROP2, TACD2, M1S1, GP50, GA733-1, GA7331, EGP-1, EGP1) Copy Product Info

Synonyms: tumor-associated calcium signal transducer 2, TROP-2, TROP2, TACD2, M1S1, GP50, GA733-1, GA7331, EGP-1, EGP1

Catalog No. TMPY-01262 Copy Product Info

TROP-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 29.4 kDa and the accession number is P09758.

Pack Size	Price	USA Stock	Global Stock
5 µg	Preferential	-	In Stock
10 µg	$38	-	In Stock
20 µg	$57	-	In Stock
50 µg	$105	7-10 days	7-10 days
100 µg	$172	In Stock	-

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 94.3%; SEC-HPLC: 91.9%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Immobilized Anti-TROP2 Antibody at 2μg/mL (100 μL/well) can bind Recombinant Human TROP2/TACSTD2 Protein (His Tag) , the EC50 is 1.6-4.8 ng/mL. 2. Loaded Anti-Human TROP2 antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human TROP2 Protein, His Tag with an affinity constant of 1.64 nM as determined in BLI assay (Routinely tested).
Description	TROP-2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 29.4 kDa and the accession number is P09758.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P09758
Construction	A DNA sequence encoding the extracellular domain (Met1-Thr274) of human TROP2 (NP_002344.2) was expressed fused with a polyhistidine tag at the C-terminus. Predicted N terminal: His 27
Protein Purity	> 96 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	tumor-associated calcium signal transducer 2, TROP-2, TROP2, TACD2, M1S1, GP50, GA733-1, GA7331, EGP-1, EGP1
Research Background	TROP-2, also referred to as tumor-associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, which are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.

Chemical Properties

Molecular Weight

29.4 kDa (predicted); 42-48 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.