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Anti-Caspase-3 Antibody (9L259)
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Catalog No. TMAH-00140
Alias Yama, SCA-1, CPP32B, CPP32, apopain
Anti-Caspase-3 Antibody (9L259) is an antibody targeting Caspase-3. Anti-Caspase-3 Antibody (9L259) can be used in ELISA,IHC,WB.
Anti-Caspase-3 Antibody (9L259)
Anti-Caspase-3 Antibody (9L259)
😃Good
Catalog No. TMAH-00140Alias Yama, SCA-1, CPP32B, CPP32, apopain
Anti-Caspase-3 Antibody (9L259) is an antibody targeting Caspase-3. Anti-Caspase-3 Antibody (9L259) can be used in ELISA,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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| Description | Anti-Caspase-3 Antibody (9L259) is an antibody targeting Caspase-3. Anti-Caspase-3 Antibody (9L259) can be used in ELISA,IHC,WB. |
| Synonyms | Yama, SCA-1, CPP32B, CPP32, apopain |
| Ig Type | Rabbit IgG |
| Clone | 9L259 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate -All lanes: Caspase-3 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 32 kDa -Observed band size: 32, 28 kDa 2. IHC image of TMAH-00140 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Caspase-3 |
| Antigen Species | Human |
| Gene Name | CASP3 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cancer, Cell biology, Metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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