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Anti-Latexin Antibody (4S702)
😃Good
Catalog No. TMAY-00473
Alias latexin
Anti-Latexin Antibody (4S702) is a Rabbit antibody targeting Latexin. Anti-Latexin Antibody (4S702) can be used in WB,ELISA,FCM,ICC/IF,IP.
Anti-Latexin Antibody (4S702)
Anti-Latexin Antibody (4S702)
😃Good
Catalog No. TMAY-00473Alias latexin
Anti-Latexin Antibody (4S702) is a Rabbit antibody targeting Latexin. Anti-Latexin Antibody (4S702) can be used in WB,ELISA,FCM,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Latexin Antibody (4S702) is a Rabbit antibody targeting Latexin. Anti-Latexin Antibody (4S702) can be used in WB,ELISA,FCM,ICC/IF,IP. |
| Alias | latexin |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 4S702 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human LXN expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LXN, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Anti-LXN rabbit monoclonal antibody at 1:500 dilution. -Lane A: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:26 kDa. -Observed band size:26 kDa. 4. LXN was immunoprecipitated using: -Lane A:0.5 mg A549 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -2 µL anti-LXN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-LXN rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25.7 kDa. -Observed band size: 25.7 kDa |
| Application | WB,ELISA,FCM,ICC/IF,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; IP: 4-6 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Latexin / LXN (rh Latexin / LXN; TMPY-00870; NP_064554.3; Glu 2-Glu 222) . |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin / LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Latexin / LXN Protein (TMPY-00870) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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