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Anti-PCNA Antibody (7I218)

😃Good
Catalog No. TMAY-02250
Alias proliferating cell nuclear antigen, ATLD2

Anti-PCNA Antibody (7I218) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (7I218) can be used in WB,ELISA,IHC-P.

Anti-PCNA Antibody (7I218)

Anti-PCNA Antibody (7I218)

😃Good
Catalog No. TMAY-02250Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (7I218) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (7I218) can be used in WB,ELISA,IHC-P.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PCNA Antibody (7I218) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (7I218) can be used in WB,ELISA,IHC-P.
Aliasproliferating cell nuclear antigen, ATLD2
Ig Type
Monoclonal Rabbit IgG
Clone
7I218
Reactivity
Human
Verified Activity
1. Immunochemical staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

2. Immunochemical staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

3. Immunochemical staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

4. Immunochemical staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

5. Anti-PCNA rabbit polyclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: MCF-7 Whole Cell Lysate.

-Lane C: MOLT-4 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:29 kDa.

-Observed band size:35 kDa
Application
WB,ELISA,IHC-P
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human PCNA (rh PCNA; TMPY-02543; P12004; Met1-Ser261).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundProliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human PCNA Protein (TMPY-02543)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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