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Anti-PCNA Antibody (7I218)
😃Good
Catalog No. TMAY-02250
Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (7I218) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (7I218) can be used in WB,ELISA,IHC-P.
Anti-PCNA Antibody (7I218)
Anti-PCNA Antibody (7I218)
😃Good
Catalog No. TMAY-02250Alias proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (7I218) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (7I218) can be used in WB,ELISA,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IHC-P Reactivity: Human |
| Alias | proliferating cell nuclear antigen, ATLD2 |
| Ig Type | Rabbit IgG |
| Clone | 7I218 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 2. Immunochemical staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 4. Immunochemical staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 5. Anti-PCNA rabbit polyclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: MCF-7 Whole Cell Lysate. -Lane C: MOLT-4 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:29 kDa. -Observed band size:35 kDa |
| Application | WB,ELISA,IHC-P |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human PCNA (rh PCNA; TMPY-02543; P12004; Met1-Ser261). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human PCNA Protein (TMPY-02543) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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