Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | In stock | $ 442.00 | |
200 μg | 5 days | $ 706.00 | |
500 μg | 5 days | $ 1,310.00 |
Biological Information |
1. Immobilized Recombinant Human IL6 / Interleukin-6 Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human IL6R / CD126 Protein (His Tag) , the EC50 is 8-24 ng/mL.
2. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 20-80 ng/mL.
3. Using the Octet RED System, the affinity constant (Kd) of IL-6R Protein, Human, Recombinant (His Tag) bound Anti-IL6R Antibody was 0.1 nM.
4.Loaded Recombinant Human IL-6R Protein, His Tag on His1K Biosensor, can bind Recombinant Human IL-6 Protein, no Tag with an affinity constant of 0.165 µM as determined in BLI assay(Routinely tested).
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Description | Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
Species | Human |
Expression System | HEK293 |
Tag | His |
Accession Number | A0N0L5 |
Synonyms | IL-6R-1, IL-6RA, IL6Q, CD126, interleukin 6 receptor, IL6RA, IL6RQ, IL-6R, gp80 |
Construction | A DNA sequence encoding the extracellular domain of human IL6R (NP_000556.1) corresponding to amino acid (Met1-Pro365) was expressed with a C-terminal polyhistidine tag. |
Protein Purity |
≥ 90 % as determined by SDS-PAGE ≥ 95 % as determined by SEC-HPLC.
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Molecular Weight | Approxiamtely 40 kDa |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Reconstitution | A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information. |
Stability & Storage |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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Please read the User Guide of Recombinant Proteins for more specific information.
IL-6R Protein, Human, Recombinant (His) IL-6R-1 IL-6RA IL6Q CD126 interleukin 6 receptor CD-126 IL6RA CD 126 IL6RQ IL-6R gp80 recombinant recombinant-proteins proteins protein