1.Immobilized Recombinant Human DLL4/Delta4 Protein (His Tag) (Cat#TMPY-01321) at 2 μg/mL (100 μL/well) can bind Recombinant Human NOTCH1 Protein (Fc Tag) (Cat#TMPY-05081), the EC50 is 20-60 ng/mL.
2.Loaded Recombinant Human NOTCH1 Protein, hFc Tag (Cat#TMPY-05081) on ProA Biosensor, can bind Recombinant Human DLL1 Protein, His Tag (Cat#TMPY-01379) with an affinity constant of 0.889 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
3.Loaded Recombinant Human NOTCH1 Protein, hFc Tag (Cat#TMPY-05081) on ProA Biosensor, can bind Recombinant Human DLL4 Protein, His Tag (Cat#TMPY-01321) with an affinity constant of 51.7nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
4.Recombinant Human NOTCH1 Protein, hFc Tag (Cat#TMPY-05081) captured on Protein A chip can bind Recombinant Human DLL4 Protein, His Tag (Cat#TMPY-01321) with an affinity constant of 1.356 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
5.Recombinant Human NOTCH1 Protein, hFc Tag (Cat#TMPY-05081) captured on Protein A chip can bind Biotinylated Recombinant Human DLL4 Protein, His & Avi Tag (Cat#TMPY-06876) with an affinity constant of 0.147 μM as determined in an SPR assay (Biacore T200) (Routinely tested).

Notch 1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 79.7 kDa and the accession number is P46531.

Human

HEK293 Cells

TAN1,notch 1,hN1,AOVD1,AOS5

A DNA sequence encoding the human NOTCH1 (NP_060087.3) (Ala19-Gln526) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Ala 19

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair the regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL). The NOTCH signaling pathway is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems. The proto-oncogene NOTCH1 is frequently mutated in around 10% of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand-binding activity. When NOTCH1 is activated by ligand binding, the NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane.