Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, FCM, IP

Reactivity: Human, Rat

Rabbit IgG

9T93

Human, Rat

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
-All lanes: Hsp90 alpha + beta antibody at 1.25μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 90 KDa
-Observed band size: 90 KDa
2. Immunoprecipitating Hsp90 alpha + beta in Hela whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00573 in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00573 (3μg) + Hela whole cell lysate (500μg)
-Lane 3: Hela whole cell lysate (20μg)
3. Overlay histogram showing Jurkat cells stained with TMAH-00573 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FCM, IP

WB:1:500-1:5000; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human HSP90AA1/HSP90AB1

Human

Signal Transduction