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Anti-Laminin subunit beta-1 Polyclonal Antibody

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Catalog No. TMAB-01044
Alias Laminin-8 subunit beta, Laminin-6 subunit beta, Laminin-2 subunit beta, Laminin-12 subunit beta, Laminin-10 subunit beta, Laminin-1 subunit beta, Laminin subunit beta 1, Laminin B1 chain, LAMB1

Anti-Laminin subunit beta-1 Polyclonal Antibody is a Rabbit antibody targeting Laminin subunit beta-1. Anti-Laminin subunit beta-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-Laminin subunit beta-1 Polyclonal Antibody

Anti-Laminin subunit beta-1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01044Alias Laminin-8 subunit beta, Laminin-6 subunit beta, Laminin-2 subunit beta, Laminin-12 subunit beta, Laminin-10 subunit beta, Laminin-1 subunit beta, Laminin subunit beta 1, Laminin B1 chain, LAMB1
Anti-Laminin subunit beta-1 Polyclonal Antibody is a Rabbit antibody targeting Laminin subunit beta-1. Anti-Laminin subunit beta-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-Laminin subunit beta-1 Polyclonal Antibody is a Rabbit antibody targeting Laminin subunit beta-1. Anti-Laminin subunit beta-1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
AliasLaminin-8 subunit beta, Laminin-6 subunit beta, Laminin-2 subunit beta, Laminin-12 subunit beta, Laminin-10 subunit beta, Laminin-1 subunit beta, Laminin subunit beta 1, Laminin B1 chain, LAMB1
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Cow)
Verified Activity
1. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: Transplantation tumor of In nude mice; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-laminin Polyclonal Antibody, Unconjugated (TMAB-01044) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Paraformaldehyde-fixed, paraffin embedded (human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (TMAB-01044) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (TMAB-01044) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (laminin) Polyclonal Antibody, Unconjugated (TMAB-01044) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
8. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Skin (Mouse) Lysate at 40 μg
Lane 3: Placenta (Mouse) Lysate at 40 μg
Lane 4: U2os (Human) Cell Lysate at 30 μg
Lane 5: Huvec (Human) Cell Lysate at 30 μg
Lane 6: HepG2 (Human) Cell Lysate at 30 μg
Lane 7: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 8: A549 (Human) Cell Lysate at 30 μg
Lane 9: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-laminin (TMAB-01044) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 220-240 kDa
Observed band size: 240 kDa
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular Localizationreted, extracellular space, extracellular matrix, basement membrane<br>Note: Major component.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundLaminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis. [provided by RefSeq, Aug 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Laminin subunit beta-1
Antigen Species
Human
Gene ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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