B7-2/CD86 Protein, Mouse, Recombinant (His)

Name: B7-2/CD86 Protein, Mouse, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01206
Rating: 4.5 (1 reviews)

(Synonyms: TS/A-2, MB7-2, MB7, Ly-58, Ly58, ETC-1, CLS1, CD86 antigen, Cd28l2, B7-2, B70, B7.2, B7) Copy Product Info

Synonyms: TS/A-2, MB7-2, MB7, Ly-58, Ly58, ETC-1, CLS1, CD86 antigen, Cd28l2, B7-2, B70, B7.2, B7

Catalog No. TMPY-01206 Copy Product Info

CD86 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.19 kDa and the accession number is P42082-1.

B7-2/CD86 Protein, Mouse, Recombinant (His)

Pack Size	Price	USA Stock	Global Stock
5 µg	$44	7-10 days	7-10 days
10 µg	$68	7-10 days	7-10 days
20 µg	$108	7-10 days	7-10 days
50 µg	$215	7-10 days	7-10 days
100 µg	$413	7-10 days	7-10 days
200 µg	$649	7-10 days	7-10 days
500 µg	$1,380	7-10 days	7-10 days
1 mg	$2,990	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Mouse B7-2, His Tag at 1μg/ml (100 μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA. (QC Test) Loaded Human CTLA-4, hFc Tag on ProA-Biosensor can bind Mouse B7-2, His Tag with an affinity constant of 0.83 µM as determined in BLI assay .
Description	CD86 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.19 kDa and the accession number is P42082-1.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P42082-1
Construction	Val24-Lys244
Protein Purity	> 95% as determined by Bis-Tris PAGE, > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	TS/A-2, MB7-2, MB7, Ly-58, Ly58, ETC-1, CLS1, CD86 antigen, Cd28l2, B7-2, B70, B7.2, B7
Research Background	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.

Chemical Properties

Molecular Weight

26.19 kDa (predicted); 48-68 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.