Immobilized Mouse B7-2, His Tag at 1μg/ml (100 μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA. (QC Test)
Loaded Human CTLA-4, hFc Tag on ProA-Biosensor can bind Mouse B7-2, His Tag with an affinity constant of 0.83 µM as determined in BLI assay .

CD86 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.19 kDa and the accession number is P42082-1.

Mouse

HEK293 Cells

TS/A-2,MB7-2,MB7,Ly-58,Ly58,ETC-1,CLS1,CD86 antigen,Cd28l2,B7-2,B70,B7.2,B7

Val24-Lys244

> 95% as determined by Bis-Tris PAGE, > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.