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Anti-His Tag Antibody (8J279)
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Catalog No. TMAB-00862
Alias HisX6, Histidine, His-Tag, HHHHHH tag, HHHHHH epitope tag, Hexa His tag, 6His, 6 His epitope tag
Anti-His Tag Antibody (8J279) is a Mouse antibody targeting His Tag. Anti-His Tag Antibody (8J279) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
Anti-His Tag Antibody (8J279)
Anti-His Tag Antibody (8J279)
😃Good
Catalog No. TMAB-00862Alias HisX6, Histidine, His-Tag, HHHHHH tag, HHHHHH epitope tag, Hexa His tag, 6His, 6 His epitope tag
Anti-His Tag Antibody (8J279) is a Mouse antibody targeting His Tag. Anti-His Tag Antibody (8J279) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $108 | 7-10 days | 7-10 days | |
| 200 μL | $184 | 7-10 days | 7-10 days | |
| 500 μL | $428 | 7-10 days | 7-10 days | |
| 1 mL | $806 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-His Tag Antibody (8J279) is a Mouse antibody targeting His Tag. Anti-His Tag Antibody (8J279) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Synonyms | HisX6, Histidine, His-Tag, HHHHHH tag, HHHHHH epitope tag, Hexa His tag, 6His, 6 His epitope tag |
| Ig Type | IgG |
| Clone | 8J279 |
| Reactivity | Species independent |
| Verified Activity | 50 ng Recombinant GFP protein per Lane probed with His tag monoclonal antibody respectively, unconjugated (TMAB-00862) at 1:5000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at RT for 60 min.  |
| Application | |
| Recommended Dose | ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:5000-50000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Immunogen | KLH conjugated HHHHHH peptide |
| Protein Name | His tag |
| Biology Area | 6x his tag®,His Tag |
| Color | Transparent |
| Appearance | Liquid |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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