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VEGF165 Protein, Human, Recombinant

Catalog No. TMPJ-00864
Synonyms: Vascular Endothelial Growth Factor Isoform 165, VEGF165

Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

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VEGF165 Protein, Human, Recombinant
Pack Size Availability Price/USD Quantity
10 μg In stock $ 118.00
50 μg 5 days $ 350.00
500 μg 5 days $ 1,390.00
1 mg 5 days $ 3,100.00
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Biological Description
Technical Params
Product Properties
Biological Information 1. Loaded Human VEGFR1-Fc on Protein A Biosensor, can bind Human VEGF165 with an affinity constant of 0.29 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human VEGF165 at 2μg/ml (100 μl/well) can bind Human VEGF165 antibody. The ED50 of Human VEGF165 antibody is 1.44 ng/ml. (Regularly tested)

Description Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.
Species Human
Expression System HEK293 Cells
Tag Tag Free
Accession Number P15692-4
Synonyms Vascular Endothelial Growth Factor Isoform 165, VEGF165
Amino Acid Ala27-Arg191
Construction Ala27-Arg191
Protein Purity Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

Molecular Weight 18-22 KDa (reducing condition)
Endotoxin < 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Formulation Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH4.0.
Reconstitution Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Shipping

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

Research Background Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling.

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Keywords

VEGF165 Protein, Human, Recombinant Vascular Endothelial Growth Factor Isoform 165 VEGF 165 VEGF-165 VEGF165 recombinant recombinant-proteins proteins protein

 

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