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Anti-DARS Antibody (5Z51)
😃Good
Catalog No. TMAY-02711
Alias HBSL, aspRS, aspartyl-tRNA synthetase
Anti-DARS Antibody (5Z51) is a Mouse antibody targeting DARS. Anti-DARS Antibody (5Z51) can be used in ELISA,FCM,ICC/IF.
Anti-DARS Antibody (5Z51)
Anti-DARS Antibody (5Z51)
😃Good
Catalog No. TMAY-02711Alias HBSL, aspRS, aspartyl-tRNA synthetase
Anti-DARS Antibody (5Z51) is a Mouse antibody targeting DARS. Anti-DARS Antibody (5Z51) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, FCM, ICC/IF Reactivity: Human |
| Alias | HBSL, aspRS, aspartyl-tRNA synthetase |
| Ig Type | Mouse IgG1 |
| Clone | 5Z51 |
| Reactivity | Human |
| Verified Activity | 1. Flow cytometric analysis of Human DARS expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human DARS, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of Human DARS in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human DARS monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm. |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human DARS (rh DARS; TMPY-03393; P14868; Met1-Pro501). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Aspartate tRNA ligase 1, also known as DARS, is part of a multienzyme complex of aminoacyl-tRNA synthetases. It belongs to the class-II aminoacyl-tRNA synthetase family. DARS charges its cognate tRNA with aspartate during protein biosynthesis. DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid(AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human DARS protein (TMPY-03393) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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