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Anti-N Cadherin Antibody (3E575)
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Catalog No. TMAY-01507
Alias N-cadherin, Ncad, CDHN, cadherin 2, type 1, N-cadherin (neuronal)
Anti-N Cadherin Antibody (3E575) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (3E575) can be used in WB,ELISA,FCM,ICC/IF.
Anti-N Cadherin Antibody (3E575)
Anti-N Cadherin Antibody (3E575)
😃Good
Catalog No. TMAY-01507Alias N-cadherin, Ncad, CDHN, cadherin 2, type 1, N-cadherin (neuronal)
Anti-N Cadherin Antibody (3E575) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (3E575) can be used in WB,ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $270 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-N Cadherin Antibody (3E575) is a Rabbit antibody targeting N Cadherin. Anti-N Cadherin Antibody (3E575) can be used in WB,ELISA,FCM,ICC/IF. |
| Alias | N-cadherin, Ncad, CDHN, cadherin 2, type 1, N-cadherin (neuronal) |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 3E575 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human CDH2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CDH2 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to membrane. 2. Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody [solid line, and Clone 1T666 (upper), Clone 3E575 (bottom)] at matching concentrations , then a FITC-conjugated second step antibody. 3. Anti-CDH2 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:99 kDa. -Observed band size:130 kDa. 4. Anti-N Cadherin rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: N Cadherin konckout 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:99 kDa. -Observed band size:130 kDa(Validation Experiment) |
| Application | WB,ELISA,FCM,ICC/IF |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human N-Cadherin / CD325 / CDH2 (rh N-Cadherin / CD325 / CDH2; TMPY-01143; NP_001783.2; Met 1-Ala 724). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Cadherins are calcium-dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass transmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression. |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Human N-Cadherin / CD325 / CDH2 Protein (TMPY-01143) |
| Antigen Species | Human |
| Biology Area | Early Mesodermal Lineage Markers |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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