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CD3D & CD3E Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.75 kDa.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $600 | 7-10 days | |
500 μg | $3,100 | 7-10 days |
Biological Activity | Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein (ECD, His Tag) at 2 μg/mL (100 μl/well) can bind CD3e/CD3 epsilon Antibody, Rabbit MAb , the EC50 is 5-15 ng/mL. |
Description | CD3D & CD3E Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.75 kDa. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | P04234-1&P07766-1 |
Synonyms | T3D,IMD19,CD3-δ,CD3-DELTA,CD3d molecule, δ (CD3-TCR complex),CD3d molecule, delta (CD3-TCR complex) |
Construction | A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766-1) constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. Predicted N terminal: Phe 22 & Asp 23 |
Protein Purity | ≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
Molecular Weight | 22.75 kDa (predicted); 28.70 kDa 、20.22 kDa and 18.06 kDa (reducing condition, due to glycosylation) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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