LIFR Protein, Human, Recombinant (His)

Name: LIFR Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00991
Price: 48 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: SWS, STWS, SJS2, LIF-R, leukemia inhibitory factor receptor α, leukemia inhibitory factor receptor alpha, CD118) Copy Product Info

Synonyms: SWS, STWS, SJS2, LIF-R, leukemia inhibitory factor receptor α, leukemia inhibitory factor receptor alpha, CD118

Catalog No. TMPY-00991 Copy Product Info

LIFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 91 kDa and the accession number is P42702-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	-	In Stock
10 µg	$75	-	In Stock
20 µg	$118	-	In Stock
50 µg	$229	-	In Stock
100 µg	$386	-	In Stock
200 µg	$682	7-10 days	7-10 days
500 µg	$1,430	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 95%; SEC-HPLC: 90.7%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Immobilized LIF Protein, Human, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind LIFR Protein, Human, Recombinant (His Tag), the EC50 is 60-360 ng/mL.
Description	LIFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 91 kDa and the accession number is P42702-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P42702-1
Construction	A DNA sequence encoding the extracellular domain of human LIFR (NP_001121143.1) (Met 1-Ser 833) with a C-terminal polyhistidine tag was expressed. Predicted N terminal: Gln 45
Protein Purity	≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	SWS, STWS, SJS2, LIF-R, leukemia inhibitory factor receptor α, leukemia inhibitory factor receptor alpha, CD118
Research Background	LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine). Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

Chemical Properties

Molecular Weight

91 kDa (predicted); 125-135 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.