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F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP
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Catalog No. TMAB-02062H
Alias Peroxidase AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L), HRP AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L), F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (HRP), F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/HRP, F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (HRP)
F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP is a HRP-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP can be used in ELISA,IHC-F,IHC-P,WB.
F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP
F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP
😃Good
Catalog No. TMAB-02062HAlias Peroxidase AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L), HRP AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L), F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (HRP), F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/HRP, F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (HRP)
F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP is a HRP-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP can be used in ELISA,IHC-F,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $225 | 7-10 days |
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Chemical Properties
| Description | F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP is a HRP-conjugated Goat antibody targeting IgG H&L. F(ab')₂ Fragment Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP can be used in ELISA,IHC-F,IHC-P,WB. |
| Synonyms | Peroxidase AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG (H+L), HRP AffiniPure F(ab')2 Fragment Goat Anti-Rabbit IgG (H+L), F(ab')₂ Fragment Goat Anti-rabbit IgG H+L (HRP), F(ab)2 Fragment Goat Anti-Rabbit IgG H&L/HRP, F(ab)2 Fragment Goat Anti-Rabbit IgG H&L (HRP) |
| Ig Type | IgG |
| Reactivity | Rabbit |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02062H 1:300) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GFAP) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02062H 1:300) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02062H 1:300) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02062H 1:300) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H3 (Nuclear Loading Control)) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02062H 1:300) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucagon) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02062H 1:300) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ACE2) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) (TMAB-02062H 1:300) instructionsand DAB staining.        |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:400-800; ELISA: 1:5000-10000; IHC-F: 1:100-1000 |
| Antibody Type | Polyclonal |
| Host Species | Goat |
| Construction | Polyclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| Concentration | 1.0 mg/ml |
| Research Background | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| Immunogen | Native Rabbit IgG |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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