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Anti-Phospho-BRAF (Thr401) Antibody (2Z641)
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Catalog No. TMAH-00884
Alias v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p-BRAF (Thr401), p-BRAF (T401), p94, EC 2.7.11.1, BRAF1, BRAF (p-Thr401), BRAF (p-T401)
Anti-Phospho-BRAF (Thr401) Antibody (2Z641) is an antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (2Z641) can be used in ELISA, WB, IF.
Anti-Phospho-BRAF (Thr401) Antibody (2Z641)
Anti-Phospho-BRAF (Thr401) Antibody (2Z641)
😃Good
Catalog No. TMAH-00884Alias v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p-BRAF (Thr401), p-BRAF (T401), p94, EC 2.7.11.1, BRAF1, BRAF (p-Thr401), BRAF (p-T401)
Anti-Phospho-BRAF (Thr401) Antibody (2Z641) is an antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (2Z641) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Alias | v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p-BRAF (Thr401), p-BRAF (T401), p94, EC 2.7.11.1, BRAF1, BRAF (p-Thr401), BRAF (p-T401) |
| Ig Type | Rabbit IgG |
| Clone | 2Z641 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in A549 whole cell lysate(treated with EGF or not) -All lanes Phospho-BRAF antibody at 1.3μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 85 KDa -Observed band size: 85 KDa 2. Immunofluorescence staining of HepG2 cells with TMAH-00884 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. Cell membrane. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-BRAF (T401) |
| Antigen Species | Human |
| Gene ID | 673 |
| Uniprot ID | |
| Biology Area | Cell Biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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