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Anti-Phospho-BRAF (Thr401) Antibody (2Z641) (Synonyms: v-Raf murine sarcoma viral oncogene homolog B1, Serine/t...)

Catalog No. TMAH-00884 Copy Product Info
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Anti-Phospho-BRAF (Thr401) Antibody (2Z641) is an antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (2Z641) can be used in ELISA,IF,WB.

Anti-Phospho-BRAF (Thr401) Antibody (2Z641)

Copy Product Info
🥰Excellent
Catalog No. TMAH-00884
Synonyms v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p-BRAF (Thr401), p-BRAF (T401), p94, EC 2.7.11.1, BRAF1, BRAF (p-Thr401), BRAF (p-T401)

Anti-Phospho-BRAF (Thr401) Antibody (2Z641) is an antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (2Z641) can be used in ELISA,IF,WB.

Anti-Phospho-BRAF
(Thr401) Antibody
(2Z641)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2097-10 days7-10 days
100 μL$3487-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-BRAF (Thr401) Antibody (2Z641) is an antibody targeting Phospho-BRAF (Thr401). Anti-Phospho-BRAF (Thr401) Antibody (2Z641) can be used in ELISA,IF,WB.
Synonymsv-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf, RAFB1, Proto-oncogene B-Raf, Phospho-BRAF (T401), p-BRAF (Thr401), p-BRAF (T401), p94, EC 2.7.11.1, BRAF1, BRAF (p-Thr401), BRAF (p-T401)
Ig Type
Rabbit IgG
Clone
2Z641
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in A549 whole cell lysate(treated with EGF or not)
-All lanes Phospho-BRAF antibody at 1.3μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 85 KDa
-Observed band size: 85 KDa
2. Immunofluorescence staining of HepG2 cells with TMAH-00884 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application
Recommended Dose
WB:1:500-1:5000; IF:1:20-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus. Cytoplasm. Cell membrane.
Tissue SpecificityBrain and testis.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundProtein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway. May play a role in the postsynaptic responses of hippocampal neurons.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human Phospho-BRAF (T401)
Antigen Species
Human
Gene Name
BRAF
Gene ID
Uniprot ID
Biology Area
Cell Biology
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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