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Synonyms: CD8A&CD8B, CD8 alpha&beta


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $123 | 7-10 days | 7-10 days | |
| 10 µg | $189 | 7-10 days | 7-10 days | |
| 20 µg | $293 | 7-10 days | 7-10 days | |
| 50 µg | $548 | 7-10 days | 7-10 days | |
| 100 µg | $873 | 7-10 days | 7-10 days | |
| 200 µg | $1,390 | 7-10 days | 7-10 days | |
| 500 µg | $2,580 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Biotinylated Human CD8 alphaβ, His Avi Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-CD8 alphaβ Antibody, hFc Tag with the EC50 of 4.3 ng/ml determined by ELISA (QC Test). |
| Description | CD8 alpha & beta Heterodimer Protein, Human, Recombinan (His & Avi & Flag), Biotinylated is expressed in HEK293 Cells. The accession number is P01732-1(CD8 alpha)&P10966-1(CD8 beta). |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi, C-Flag |
| Accession Number | P01732-1(CD8 alpha)&P10966-1(CD8 beta) |
| Construction | Ser22-Asp182 (CD8 alpha) acidic tail and Leu22-Pro170 (CD8 beta) basic tail |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | CD8A&CD8B, CD8 alpha&beta |
| Research Background | CD8 alphaβ (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+peripheral T cells bearing TCR. |
| Molecular Weight | 25.35 kDa (CD8 alpha) and 22.62 kDa (CD8 beta) (Predicted); 32-36 kDa and 38-45 kDa (Reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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