Others

Rift Valley fever virus (RVFV) (strain MP12) glycoprotein G1 Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 47.8 kDa and the accession number is A2T077.

Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. OSM encodes a growth regulator, which Inhibits the proliferation of a number of tumor cell lines. It stimulates proliferation of AIDS-KS cells. OSM regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is considered as a pleiotropic cytokine that initiates its biological activities through specific cell surface receptors. The low affinity LIF receptor that shares the similarity of containing protein gp130 has now been identified to be a component of a high- affinity OSM receptor that will transduce OSM signals. OSM has also been shown to play a role in both pro and anti-inflammatory actions. OSM may also be involved in many biometabolism processes including liver development, haematopoeisis, inflammation, bone formation and destruction and possibly CNS development.

IBV (strain M41) Spike glycoprotein (His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 26.8 kDa and the accession number is P12651.

Acts as a CD40 functional homolog to prevent apoptosis of infected B-lymphocytes and drive their proliferation. Functions as a constitutively active tumor necrosis factor receptor that induces the activation of several signaling pathways, including those of the NF-kappa-B family. LMP1 signaling leads to up-regulation of antiapoptotic proteins and provide growth signals in latently infected cells. Interacts with host UBE2I and subsequently affects the sumoylation state of several cellular proteins. For example, induces the sumoylation of host IRF7 thereby limiting its transcriptional activity and modulating the activation of innate immune responses. Inhibits also host IFN-alpha-stimulated STAT2 nuclear translocation and interferon-stimulated response element transcriptional activity by interacting with and inhibiting host TYK2.

Flagellin is the major structural protein monomer of bacterial flagella. Flagellin through binding to its receptor and activation of antigen presenting cells stimulates the innate and adaptive immune responses. Flagellin is used as an effective systemic or mucosal adjuvant to stimulate the immune system. Flagellin is an agonist of Toll-like receptor 5 (TLR5), a pattern recognition receptor (PRR) of the innate immune system expressed on the basolateral surface of intestinal epithelial cells and on the surface of a subset of intestinal dendritic cells. Flagellin is delivered into the cytosol of macrophages by the T3SS-1 of serotype Typhimurium, where it activates the cytosolic interleukin-1 (IL-1) converting enzyme-protease activating factor (IPAF), a nucleotide-binding and oligomerization domain-like receptor (NLR) of the innate immunesystem. Recognition of flagellin by IPAF leads to activation of the inflammasome, followed by proteolytic activation of IL-1 and IL-18.

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 31.1 kDa and the accession number is P02708.

Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1).

Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells. Rabies virus (RABV) (strain CVS-11) Glycoprotein (His & Myc & SUMO) is expressed in E. coli expression system with N-10xHis-SUMO and C-Myc tag. The predicted molecular weight is 69.7 kDa and the accession number is O92284.

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNG Protein, Human, Recombinant (E. coli, His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 29.6 kDa and the accession number is P07510.

CCHF virus (isolate C68031) Nucleoprotein NP Protein (B2M & His) is expressed in E. coli.

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNE Protein, Human, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 32.5 kDa and the accession number is Q04844.

CLEC18A Protein, Human, Recombinant (His & SUMO) is expressed in E. coli.

Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2 M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells.

Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factors in site-specific integration of the viral genome. Regulates host PKA activity by interacting with host PRKX as a mechanism of interfering with helper virus propagation and promoting its own replication. Inhibits the host cell cycle G1 S, S and G2 M transitions. These arrests may provide essential cellular factors for viral DNA replication.

Human cytomegalovirus (HCMV) (strain AD169) Uncharacterized protein UL128 (His) is expressed in E. coli.

Human Papilloma Virus type 16 (HPV 16) Protein E6 (E. coli, His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 23.2 kDa and the accession number is P03126.

Vaccinia virus (VACV) (strain Copenhagen) Protein B5 (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 30.53 kDa and the accession number is P21115.

Osteopontin Protein, Human, Recombinant (aa 17-166, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 20.64 kDa. and the accession number is P10451.

May act as transcriptional regulator. Induces apoptosis in infected cells. Element of infectious replication cycle. Chicken anemia virus (isolate USA 26p4) Apoptin Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 17.1 kDa and the accession number is P54095.

Zika virus (ZIKV) (strain Zika SPH2015) E Envelope protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 45.5 kDa and the accession number is ALU33341.1.

Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. A29 Protein, Monkeypox virus, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 13.65 kDa and the accession number is Q90188.

Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. A35R Protein, Monkeypox virus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 14.77 kDa and the accession number is QJQ40286.1.

Simian immunodeficiency virus (SIV) (isolate 216.94.A2) gp120 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60 kDa and the accession number is E2EXP2.

The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a FlaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the FlaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases.

Human Papilloma Virus type 39 (HPV 39) L1 Protein-VLP is expressed in Baculovirus insect cells. The predicted molecular weight is 57 kDa.

Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E Envelope protein (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 13 kDa and the accession number is ALU33341.1.

Human Papilloma Virus type 33 (HPV 33) L1 Protein-VLP is expressed in Baculovirus insect cells. The predicted molecular weight is 56 kDa.

Coxsackievirus A16 (Cox A16) (strain G-10) VP1 Protein (Fc) is expressed in Baculovirus insect cells with Fc tag. The predicted molecular weight is 59.5 kDa and the accession number is AAA50478.1.

Coxsackievirus A16 (Cox A16) (strain G-10) VP4 Protein (Fc) is expressed in Baculovirus insect cells with Fc tag. The predicted molecular weight is 32.6 kDa and the accession number is AAA50478.1.

Zika virus (ZIKV) infection causes microcephaly and has been linked to other brain abnormalities. ZIKV has a more selective and larger impact on the expression of genes involved in DNA replication and repair. P53 inhibitors can block the apoptosis induced by ZIKV-M in hNPCs.

Human metapneumovirus (strain HMPV Homo sapiens PER CFI0466 2010 B) glycoprotein G Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 21.6 kDa and the accession number is X4Y8Q3.

Human Papilloma Virus type 56 (HPV 56) L1 Protein-VLP is expressed in Baculovirus insect cells. The predicted molecular weight is 56 kDa.

Human Papilloma Virus type 59 (HPV 59) L1 Protein-VLP is expressed in Baculovirus insect cells. The predicted molecular weight is 57 kDa.

Monkeypox Virus (MPXV) L1R Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 19.64 kDa.

Monkeypox Virus (MPXV) C19L Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 43.25 kDa and the accession number is QNP13638.1.

Varicella-zoster virus (VZV) (strain Oka vaccine) gH & gL Heterodimer Protein is expressed in CHO mammalian cells. The predicted molecular weight is 85.17 kDa.

Canine distemper virus (strain Onderstepoort) Fusion glycoprotein F0 (His & SUMO) is expressed in E. coli expression system with N-6xHis-SUMO tag. The predicted molecular weight is 67.5 kDa and the accession number is P12569.

IBV (strain UK 123 82) Spike glycoprotein (E. coli, His) is expressed in E. coli expression system with C-6xHis tag. The predicted molecular weight is 25.7 kDa and the accession number is P30206.

VSIV (strain San Juan) Matrix Protein (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 33.5 kDa and the accession number is P03519.

Zaire ebolavirus (strain Kikwit-95) Glycoprotein GP Protein (His & Myc) is expressed in HEK293 mammalian cells with N-10xHis and C-Myc tag. The predicted molecular weight is 34.7 kDa and the accession number is P0C773.

Rotavirus A (strain St. Thomas 3) VP4 Protein (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 28.3 kDa and the accession number is P11200.

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Acts as a functional inhibitor of gH and maintains gH in an inhibited form. Upon binding to host integrins, gL dissociates from gH leading to activation of the viral fusion glycoproteins gB and gH. Varicella-zoster virus (strain Dumas) glycoprotein L gL Protein (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 22.6 kDa and the accession number is P09308.

Zaire ebolavirus (strain Kikwit-95) VP40 Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 39.3 kDa and the accession number is Q77DJ6.

Zaire ebolavirus (strain Kikwit-95) L Protein (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 27.9 kDa and the accession number is Q6V1Q2.

Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane.; Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.; Nucleocapsid protein p13 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers. FIV (isolate Petaluma) Gag polyprotein (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 22.1 kDa and the accession number is P16087.

Encapsidates the genome protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication. Seems to participate in the nuclear relocalization of host PABP1, thereby inhibiting host cellular translation. Bunyavirus La Crosse Nucleoprotein NP Protein (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 28.5 kDa and the accession number is P04873.

Bovine viral diarrhea virus (strain SD-1) Genome polyprotein (E. coli, His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 25.0 kDa and the accession number is Q01499.