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Anti-SAE1 Antibody (3S832)
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Catalog No. TMAH-01069
Alias UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1
Anti-SAE1 Antibody (3S832) is an antibody targeting SAE1. Anti-SAE1 Antibody (3S832) can be used in ELISA,IF,WB.
Anti-SAE1 Antibody (3S832)
Anti-SAE1 Antibody (3S832)
😃Good
Catalog No. TMAH-01069Alias UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1
Anti-SAE1 Antibody (3S832) is an antibody targeting SAE1. Anti-SAE1 Antibody (3S832) can be used in ELISA,IF,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $207 | 7-10 days | 7-10 days | |
| 100 μL | $347 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SAE1 Antibody (3S832) is an antibody targeting SAE1. Anti-SAE1 Antibody (3S832) can be used in ELISA,IF,WB. |
| Synonyms | UBLE1A, SUMO1 activating enzyme subunit 1, SUA1, HSPC140, AOS1 |
| Ig Type | Rabbit IgG |
| Clone | 3S832 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate -All lanes: SAE1 Antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 39, 30, 34 kDa -Observed band size: 39 kDa 2. Immunofluorescence staining of Hela Cells with TMAH-01069 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Tissue Specificity | Expression level increases during S phase and drops in G2 phase (at protein level). |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human SAE1 |
| Antigen Species | Human |
| Gene Name | SAE1 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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