SEMA4D Protein, Human, Recombinant (His)

Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.

Pack Size	Availability	Price/USD
50 μg	In stock	$ 320.00
100 μg	5 days	$ 567.00
200 μg	5 days	$ 1,000.00
500 μg	5 days	$ 2,120.00

Pack Size

Availability

Price/USD

Quantity

50 μg

In stock

$ 320.00

100 μg

5 days

$ 567.00

200 μg

5 days

$ 1,000.00

500 μg

5 days

$ 2,120.00

Biological Information

Measured by its ability to bind mouse CD45 hFc in functional ELISA.

Description

Species	Human
Expression System	HEK293
Tag	His
Accession Number	AAH54500.1
Synonyms	CD100, COLL4, C9orf164, semaphorin 4D, SEMAJ, coll-4, M-sema-G
Construction	A DNA sequence encoding the human SEMA4D (AAH54500.1) extracellular domain (Met 1-Arg 734) was expressed, with a polyhistidine tag at the C-terminus.
Protein Purity	> 92 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 80.7 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.

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SEMA4D Protein, Human, Recombinant (His)

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