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Anti-CD86 Antibody (5D537)
😃Good
Catalog No. TMAY-03702
Alias LAB72, CD86 antigen, CD28LG2, B7-2, B70, B7.2
Anti-CD86 Antibody (5D537) is a Mouse antibody targeting CD86. Anti-CD86 Antibody (5D537) can be used in ELISA(Det),FCM.
Anti-CD86 Antibody (5D537)
Anti-CD86 Antibody (5D537)
😃Good
Catalog No. TMAY-03702Alias LAB72, CD86 antigen, CD28LG2, B7-2, B70, B7.2
Anti-CD86 Antibody (5D537) is a Mouse antibody targeting CD86. Anti-CD86 Antibody (5D537) can be used in ELISA(Det),FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 20 μL | $70 | 7-10 days | 7-10 days | |
| 100 μL | $199 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD86 Antibody (5D537) is a Mouse antibody targeting CD86. Anti-CD86 Antibody (5D537) can be used in ELISA(Det),FCM. |
| Synonyms | LAB72, CD86 antigen, CD28LG2, B7-2, B70, B7.2 |
| Ig Type | Mouse IgG2b |
| Clone | 5D537 |
| Reactivity | Rat |
| Specificity | Rat B7-2/CD86 |
| Verified Activity | Flow cytometric analysis of rat CD86 expression on LPS-stimulated SD rat splenocytes. Cells were stained with purified anti-rat CD86, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | |
| Recommended Dose | FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD86 / B7-2 (rR CD86 / B7-2; TMPY-02016; NP_064466.1; Met1-Ile250). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, 0.02% Tween 80, pH7.0 |
| Research Background | CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. A recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/HRP/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Rat CD86 / B7-2 Protein (TMPY-02016) |
| Antigen Species | Rat |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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