FCGRT & B2M Heterodimer Protein, Human, Recombinant (His)

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 cells.

Pack Size	Availability	Price/USD
100 μg	In stock	$ 473.00
200 μg	5 days	$ 834.00
500 μg	5 days	$ 1,760.00

Pack Size

Availability

Price/USD

Quantity

100 μg

In stock

$ 473.00

200 μg

5 days

$ 834.00

500 μg

5 days

$ 1,760.00

Biological Information

1. Measured by its ability to bind human IgG1 in a functional ELISA.
2. Loaded Human FcRn recombinant protein on His1K Biosensor, can bind IgG1 with an affinity constant of 0.11 µM as determined in a BLI assay.
3. Loaded Human FcRn recombinant protein on His1k Biosensor, can bind IgG4 Fc with an affinity constant of 0.08 µM as determined in a BLI assay.
4. Captured Human FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.11 µM as determined in an SPR assay (Biacore T200).
5. Captured Human FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.23 µM as determined in an SPR assay (Biacore T200).
6. The purity of Human FCGRT & B2M Heterodimer Protein is more than 90% and the molecular weight of this protein is around 45-53 kDa verified by SEC-MALS.
7. Loaded Recombinant Human FCGRT & B2M Heterodimer Protein, His Tag on His1K Biosensor, can bind Anti-Erbb2 Antibody, human IgG1 with an affinity constant of 11.1nM as determined in BLI assay (QC tested).

Description

FCGRT & B2M Heterodimer Protein, Human, Recombinant (His) is expressed in HEK293 cells.

Species	Human
Expression System	HEK293
Tag	His
Accession Number	P55899-1&P61769
Synonyms	Fc fragment of IgG, receptor, transporter, alpha, alpha-chain, α-chain, Fc fragment of IgG, receptor, transporter, α, FCRN
Construction	A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899-1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified.
Protein Purity	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Molecular Weight	Approxiamtely 43.5 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

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FCGRT & B2M Heterodimer Protein, Human, Recombinant (His)

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