Antibody Type: Mouse Monoclonal

Application: ELISA, IHC, IF

Reactivity: Human

IgG2b

9G855

Human

1. Immunofluorescence staining of U87 cells with TMAH-00477 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
2. IHC image of TMAH-00477 diluted at 1:100 and staining in paraffin-embedded human colon tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

ELISA, IHC, IF

Monoclonal

Unconjugated

Recombinant Protein: Human Guanylate-binding Protein 2 Protein (100-259AA)

Human

2634

Immunology