Anti-Phospho-SHC1 (Tyr317) Polyclonal Antibody is a Rabbit antibody targeting Phospho-SHC1 (Tyr317). Anti-Phospho-SHC1 (Tyr317) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse

1. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SHC (Tyr317))) Polyclonal Antibody, Unconjugated (TMAB-11278) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Human Jurkat cell lysates
Lane 4: Human HepG2 cell lysates
Lane 5: Human A431 cell lysates
Lane 6: Human SH-SY5Y cell lysates
Lane 7: Human HUVEC cell lysates
Primary: Anti-phospho-SHC1 (Tyr317) (TMAB-11278) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 52 kDa

WB: 1:500-2000; IHC-P: 1:50-300; IHC-Fr: 1:50-300; IF: 1:50-300; ELISA: 1:5000-10000

Polyclonal

KLH conjugated Synthesised phosphopeptide: human SHC around the phosphorylation site of Tyr317

Human

SHC1

SHC-transforming protein 1

Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.