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Anti-Strep Tag II Antibody (4T542) is a Mouse antibody targeting Strep Tag II. Anti-Strep Tag II Antibody (4T542) can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
| Description | Anti-Strep Tag II Antibody (4T542) is a Mouse antibody targeting Strep Tag II. Anti-Strep Tag II Antibody (4T542) can be used in WB. |
| Synonyms | Strep-Tag-II, Strep.Tag, Strep Tag II, Strep |
| Ig Type | IgG |
| Clone | 4T542 |
| Reactivity | Species independent |
| Verified Activity | Sample: Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysates Lane 2: Negative control Primary: Anti-Strep-Tag II/HRP (TMAB-01794-HRP) at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa ![]() |
| Application | |
| Recommended Dose | WB: 1:1000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. |
| Immunogen | KLH conjugated synthetic peptide Strep-Tag |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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