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Anti-Strep Tag II Antibody (4T542)
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Catalog No. TMAB-01794
Alias Strep-Tag-II, Strep.Tag, Strep Tag II, Strep
Anti-Strep Tag II Antibody (4T542) is a Mouse antibody targeting Strep Tag II. Anti-Strep Tag II Antibody (4T542) can be used in WB.
Anti-Strep Tag II Antibody (4T542)
Anti-Strep Tag II Antibody (4T542)
😃Good
Catalog No. TMAB-01794Alias Strep-Tag-II, Strep.Tag, Strep Tag II, Strep
Anti-Strep Tag II Antibody (4T542) is a Mouse antibody targeting Strep Tag II. Anti-Strep Tag II Antibody (4T542) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Strep Tag II Antibody (4T542) is a Mouse antibody targeting Strep Tag II. Anti-Strep Tag II Antibody (4T542) can be used in WB. |
| Alias | Strep-Tag-II, Strep.Tag, Strep Tag II, Strep |
| Ig Type | IgG |
| Clone | 4T542 |
| Reactivity | Species independent |
| Verified Activity | 1. Sample: Strep-Tagged Fusion Protein Overexpression E.coli Lysate at 4 μg Primary: Anti-Strep-Tag II (TMAB-01794) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa 2. Sample: Lane 1: Strep-Tagged Fusion Protein Overexpression E.coli Lysates Lane 2: Negative control Primary: Anti-Strep-Tag II/HRP (TMAB-01794-HRP) at 1/10000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa |
| Application | WB |
| Recommended Dose | WB: 1:1000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins. |
| Immunogen | KLH conjugated synthetic peptide Strep-Tag |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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