Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PKM2 (Tyr105). Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM,ELISA.

IgG

Human,Rat,Rabbit (predicted:Mouse,Dog,Pig,Cow,Horse,GuineaPig)

1. phosphopeptide
non phosphopeptide
2. Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: Rat Thyroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-NMDAR1 (Ser890) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugati Phospho-PKM2on to the secondary antibody and DAb staining.
4. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Tissue/cell: rat thyroid tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
6. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
7. Tissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Phospho-PKM2 (Tyr105) Polyclonal Antibody, Unconjugated (TMAB-01482) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
8. Blank control: A549. Primary Antibody (green line): Rabbit Anti-Phospho-PKM2 (Tyr105) antibody (TMAB-01482)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

IHC-P,IHC-Fr,IF,FCM,ELISA

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated Synthesised phosphopeptide: human PKM2 around the phosphorylation site of Tyr105

Human

Energy Metabolism,Energy Metabolism,Carbohydrate metabolism,Kinases/Phosphatases,Enzymes,Metabolism of carbohydrates