VEGF165 Protein, Human/Cynomolgus, Recombinant V2

Catalog No. TMPY-01041U

VEGF165 Protein, Human/Cynomolgus, Recombinant V2 is expressed in HEK293 Cells. The accession number is P15692-4.

VEGF165 Protein, Human/Cynomolgus, Recombinant V2

Catalog No. TMPY-01041U

VEGF165 Protein, Human/Cynomolgus, Recombinant V2 is expressed in HEK293 Cells. The accession number is P15692-4.

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Biological Activity	Immobilized Human VEGF165 at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3 ng/ml determined by ELISA. Human VEGF165 stimulates proliferation of human umbilical vein endothelial cells (HUVEC) with the ED50 for this effect is 2.57 ng/mL (QC Test).
Description	VEGF165 Protein, Human/Cynomolgus, Recombinant V2 is expressed in HEK293 Cells. The accession number is P15692-4.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P15692-4
Synonyms	VPF,VEGFA,VEGF,Vascular permeability factor,L-VEGF
Construction	Ala27-Arg191
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	19.2 kDa (Predicted); 20-30 kDa (Due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.