LAP (TGF-beta 1) Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPJ-00250

Transforming growth factor beta (TGFβ) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGFβ gene product. Extracellular activation of these complexes is a critical step in regulation of TGFβ function in vivo. The TGFβ1 LAP is a ligand for the integrin αvβ6 and that αvβ6-expressing cells induce spatially restricted activition of TGFβ1. And LAP identifies a novel CD4+/CD25+ regulatory T cell subset with TGFbeta-mediated function and enhanced suppression of experimental autoimmune encephalomyelitis.

LAP (TGF-beta 1) Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPJ-00250

Pack Size	Price	Availability
5 μg	$129	7-10 days
10 μg	$213	7-10 days
20 μg	$355	7-10 days
50 μg	$625	7-10 days
100 μg	$963	7-10 days

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Product Information

Biological Activity	Immobilized Human ITGAV&ITGB6, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.11 μg/ml determined by ELISA (QC Test).
Description	Transforming growth factor beta (TGFβ) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGFβ gene product. Extracellular activation of these complexes is a critical step in regulation of TGFβ function in vivo. The TGFβ1 LAP is a ligand for the integrin αvβ6 and that αvβ6-expressing cells induce spatially restricted activition of TGFβ1. And LAP identifies a novel CD4+/CD25+ regulatory T cell subset with TGFbeta-mediated function and enhanced suppression of experimental autoimmune encephalomyelitis.
Species	Human
Expression System	HEK293 Cells
Tag	N-His-Avi
Accession Number	P01137
Synonyms	transforming growth factor β1,transforming growth factor beta 1,TGFβ,TGFbeta,TGFB1,TGFB,LAP (TGF-β 1),LAP (TGFβ 1),LAP (TGF-beta 1),LAP (TGFbeta 1),LAP,DPD1,CED
Amino Acid	Leu30-Arg278 (C33S)
Construction	Leu30-Arg278 (C33S)
Protein Purity	> 90% as determined by Bis-Tris PAGE
Molecular Weight	31.4 kDa (Predicted); 45-50 KDa (reducing condition)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Transforming growth factor beta (TGFβ) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGFβ gene product. Extracellular activation of these complexes is a critical step in regulation of TGFβ function in vivo. The TGFβ1 LAP is a ligand for the integrin αvβ6 and that αvβ6-expressing cells induce spatially restricted activition of TGFβ1. And LAP identifies a novel CD4+/CD25+ regulatory T cell subset with TGFbeta-mediated function and enhanced suppression of experimental autoimmune encephalomyelitis.