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Anti-PSME2 Antibody (7S935)
😃Good
Catalog No. TMAY-02763
Alias REGβ, REGbeta, proteasome (prosome, macropain) activator subunit 2 (PA28 β), proteasome (prosome, macropain) activator subunit 2 (PA28 beta), PA28β, PA28beta, PA28B
Anti-PSME2 Antibody (7S935) is a Mouse antibody targeting PSME2. Anti-PSME2 Antibody (7S935) can be used in WB,ELISA,IP.
Anti-PSME2 Antibody (7S935)
Anti-PSME2 Antibody (7S935)
😃Good
Catalog No. TMAY-02763Alias REGβ, REGbeta, proteasome (prosome, macropain) activator subunit 2 (PA28 β), proteasome (prosome, macropain) activator subunit 2 (PA28 beta), PA28β, PA28beta, PA28B
Anti-PSME2 Antibody (7S935) is a Mouse antibody targeting PSME2. Anti-PSME2 Antibody (7S935) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA, IP Reactivity: Human |
| Alias | REGβ, REGbeta, proteasome (prosome, macropain) activator subunit 2 (PA28 β), proteasome (prosome, macropain) activator subunit 2 (PA28 beta), PA28β, PA28beta, PA28B |
| Ig Type | Mouse IgG1 |
| Clone | 7S935 |
| Reactivity | Human |
| Verified Activity | 1. Anti-PSME2 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:27 kDa. -Observed band size:30 kDa. 2. PSME2 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-PSME2 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PSME2 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 27 kDa. -Observed band size: 27 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSME2 / PA28b (rh PSME2 / PA28b; TMPY-03610; Met1-Tyr239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | PSME2, also known as PA28b, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME2 gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human PSME2 / PA28b protein (TMPY-03610) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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