Fc epsilon RI Protein, Human, Recombinant (His)

Name: Fc epsilon RI Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-03054
Price: 386 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: α polypeptide, FcERI, FCE1A, Fc ε RI, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide) Copy Product Info

Synonyms: α polypeptide, FcERI, FCE1A, Fc ε RI, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide

Catalog No. TMPY-03054 Copy Product Info

Purity: 98.4%

Fc epsilon RI Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is P12319.

Pack Size	Price	USA Stock	Global Stock
5 µg	$48	-	In Stock
10 µg	$75	-	In Stock
20 µg	$118	-	In Stock
50 µg	$229	-	In Stock
100 µg	$386	-	In Stock
200 µg	Inquiry	7-10 days	7-10 days
500 µg	Inquiry	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:98.4%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1.Immobilized Recombinant Human FcERI/FCER1A Protein (His Tag) (Cat#TMPY-03054) at 2 μg/mL (100 μl/well) can bind Recombinant Human IgE-Fc Protein (Constant Domain 3&4, His Tag) (Cat#TMPY-05071), the EC50 is 10-30 ng/mL. 2.Loaded Recombinant Human Fc epsilon RI Protein, His Tag (Cat#TMPY-03054) on AR2G Biosensor, can bind Recombinant Human IgE Fc Protein, His Tag (Cat#TMPY-05071) with an affinity constant of 1.96 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
Description	Fc epsilon RI Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is P12319.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P12319
Construction	A DNA sequence encoding the human FCER1A (P12319) (Met1-Gln205) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Val 26
Protein Purity	> 95 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	α polypeptide, FcERI, FCE1A, Fc ε RI, Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide
Research Background	FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.

Chemical Properties

Molecular Weight

22.5 kDa (predicted); 42-48 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.