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Anti-GABRA5 Antibody (6R526)

😃Good
Catalog No. TMAH-00471
Alias Gamma-aminobutyric acid receptor subunit alpha-5, GABRA 5, GABA(A) receptor subunit alpha-5

Anti-GABRA5 Antibody (6R526) is an antibody targeting GABRA5. Anti-GABRA5 Antibody (6R526) can be used in ELISA, WB, IHC.

Anti-GABRA5 Antibody (6R526)

Anti-GABRA5 Antibody (6R526)

😃Good
Catalog No. TMAH-00471Alias Gamma-aminobutyric acid receptor subunit alpha-5, GABRA 5, GABA(A) receptor subunit alpha-5
Anti-GABRA5 Antibody (6R526) is an antibody targeting GABRA5. Anti-GABRA5 Antibody (6R526) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Anti-GABRA5 Antibody (6R526) is an antibody targeting GABRA5. Anti-GABRA5 Antibody (6R526) can be used in ELISA, WB, IHC.
AliasGamma-aminobutyric acid receptor subunit alpha-5, GABRA 5, GABA(A) receptor subunit alpha-5
Ig Type
Rabbit IgG
Clone
6R526
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: Ntera-2 whole cell lysate, HEK293 whole cell lysate
-All lanes: GABA A Receptor alpha 5 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 53 kDa
-Observed band size: 70 kDa
2. IHC image of TMAH-00471 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundLigand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapse.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human GABA A Receptor alpha 5
Antigen Species
Human
Gene ID
2558
Uniprot ID
Biology Area
Neuroscience
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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