Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 581.00 | |
500 μg | 5 days | $ 2,330.00 | |
1 mg | 5 days | $ 3,880.00 |
Description | Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone. |
Species | Human |
Expression System | HEK293 |
Tag | No Tag |
Accession Number | O14788-2 |
Synonyms | OPGL, TRANCE, OPTB2, TNFSF11, ODF, sOdf, RANKL, CD254 |
Construction | Recombinant Human RANKL/TNFSF11/CD254 Protein is expressed from HEK293 without tag. It contains Gly63-Asp244.[Accession |O14788-2] |
Protein Purity | > 90% as determined by Tris-Bis PAGE; > 90% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
RANKL/TNFSF11/CD254 Protein, Human, Recombinant (aa 63-244) TNFSF 11 OPGL OPTB-2 TRANCE OPTB2 TNFSF11 OPTB 2 ODF CD 254 CD-254 sOdf RANKL CD254 TNFSF-11 recombinant recombinant-proteins proteins protein