Anti-SLC10A2 Polyclonal Antibody 3 is a Rabbit antibody targeting SLC10A2. Anti-SLC10A2 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse (predicted:Rat)

1. Measured by its binding ability in a indirect ELISA. Immobilized Mouse SLC10A2 protein, His Tag at 2 μg/mL (100 μL/well) can bind  Rabbit Anti-Mouse SLC10A2 Antibody, the EC50 is 179.9 ng/mL.
2. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SLC10A2) Polyclonal Antibody, Unconjugated (TMAB-12836) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Human SW480 cell lysates
Lane 3: Human 293T cell lysates
Primary: Anti-SLC10A2 (TMAB-12836) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 46 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: mouse SLC10A2 protein

Mouse

SLC10A2

Ileal sodium/bile acid cotransporter

Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism.