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Anti-PDK1 Monoclonal Antibody (6Y935)

(Synonyms: PDK1) Copy Product Info
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Synonyms: PDK1

Catalog No. TMAC-03137 Copy Product Info
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Anti-PDK1 Monoclonal Antibody (6Y935) is a Mouse antibody targeting PDK1. Anti-PDK1 Monoclonal Antibody (6Y935) can be used in WB,IHC,IF,FCM.

Anti-PDK1 Monoclonal Antibody
(6Y935)

Pack SizePriceUSA StockGlobal StockQuantity
50 µL$2167-10 days7-10 days
100 µL$3187-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-PDK1 Monoclonal Antibody (6Y935) is a Mouse antibody targeting PDK1. Anti-PDK1 Monoclonal Antibody (6Y935) can be used in WB,IHC,IF,FCM.
SynonymsPDK1
Ig Type
IgG1
Clone
6Y935
Reactivity
Human,Rat,Monkey
Verified Activity
1. Western Blot analysis using PDK1 Monoclonal Antibody against NIH/3T3 (1), HeLa (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.
2. Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) with DAB staining using PDK1 Monoclonal Antibody.
3. Immunofluorescence analysis of HELA cells using PDK1 Monoclonal Antibody (green).
4. Flow cytometric analysis of Lovo cells with PDK1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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Application
Recommended Dose
WB: 1/500-1/2000; IHC: 1/200-1/1000; IF: 1/200-1/1000; FCM: 1/200-1/400
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationPurified by protein A or protein G
AppearanceLiquid
FormulationPBS with 0.05% sodium azide.
Concentration1 mg/mL
Research BackgroundPyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Purified recombinant fragment of human PDK1 expressed in E. Coli.
Antigen Species
Human
Gene Name
PDK1
Gene ID
Protein Name
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 44 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: Anti-PDK1 Monoclonal Antibody (6Y935) molecular weight